plasma fgf21  (Cusabio)

Journal: medRxiv

Article Title: Elevated FGF21 and triglycerides in SLC25A13 carriers support the G3P-ChREBP Citrin Deficiency disease hypothesis

doi: 10.64898/2025.12.10.25342016

Figure Lengend Snippet: Age and ex-matched SLC25A13 mutation carriers from the TWB were assayed for FGF21 circulation and urinary sodium. The data show that male (A) and female (B) mutation carriers have higher levels of FGF21 than control participants and that male and female mutation carriers have higher levels of urinary sodium (C) than control participants. Further, female CD patients have much higher levels of circulating FGF21 than control female age-matched participants (D). (A) Plasma FGF21 levels (N = 25 per group); (B) Plasma FGF21 levels (N = 27 controls, N = 25 carriers); (C) urinary sodium (N = 48 controls, N = 49 carriers); (D) plasma FGF21 (N = 10 controls, N = 2 CD). Symbols: □ male controls; ■ male carriers; ○ female controls; ● female carriers; ● female CD patients. P values are from Student’s t-test.

Article Snippet: Plasma FGF21 levels were quantified in duplicate using the Quantikine® Human FGF-21 ELISA kit (R&D Systems, DF2100) according to the manufacturer’s protocol.

Techniques: Mutagenesis, Control, Clinical Proteomics

Journal: medRxiv

Article Title: Elevated FGF21 and triglycerides in SLC25A13 carriers support the G3P-ChREBP Citrin Deficiency disease hypothesis

doi: 10.64898/2025.12.10.25342016

Figure Lengend Snippet: (A) TGs were compared between the N = 7 control participants and the N =45 SLC25A13 mutation carriers for whom TG values were available in the TWB. These data show a nonsignificant TG increase in mutation carriers. (B) A plot of TGs as a function of FGF21 in mutation carriers shows a strong positive correlation (Pearson’s correlation R = 0.58) that is counter to the depression of TGs by genetically proxied FGF21 in the general population and consistent with elevation of FGF21 and lipogenic transcription by a potentiated ChREBP system in SLC25A13 carriers.

Article Snippet: Plasma FGF21 levels were quantified in duplicate using the Quantikine® Human FGF-21 ELISA kit (R&D Systems, DF2100) according to the manufacturer’s protocol.

Techniques: Control, Mutagenesis

Journal: Biochemical and biophysical research communications

Article Title: Growth hormone resistance induced by amino acid deprivation in fao cells is independent of FGF21.

doi: 10.1016/j.bbrc.2024.149811

Figure Lengend Snippet: Fig. 3. Effect of FGH21 addition on GH signaling. A, B) Immunoblotting of JAK2, p-JAK2, STAT5b, and HSP90. Fao cells were cultured in the Full or Zero medium with 0–10 [5] pM of recombinant FGF21 for 24 h. Cells were then treated with 100 nM GH or vehicle for 10 min. C) Igf1 mRNA levels were quantified using real-time qPCR. Values were normalized against the Actb mRNA level. Fao cells were cultured in the full medium with or without 100 nM GH and 0–105 pM of FGF21 for 24 h. Total RNA was isolated. Bar: means ± SEM (n = 3), *p < 0.05. (D) The expression of α-klotho (Kl), β-klotho (Klb), and FGF Receptor (Fgfr) 1–4 in Fao cells, rat primary hepatocytes, and rat tissue samples were analyzed using reverse transcription-PCR. (E) Immnoblotting of Erk and p-Erk. Fao cells were cultured in the full medium for 24 h. The cells were then treated with 0–104 pM of FGF21 or vehicle for 10 min.

Article Snippet: Plasma FGF21 concentrations were measured using a mouse/rat FGF21 ELISA kit (Proteintech, IL, USA).

Techniques: Western Blot, Cell Culture, Recombinant, Isolation, Expressing, Reverse Transcription